QF-Pro® Technology

Precise quantitative analysis of protein functions in patient samples

QF-Pro® is a spatial biology technology, purpose-built for quantitative functional proteomics

  • QF-Pro® is an antibody-based, two-site assay
  • It employs an enhanced and simplified FRET-FLIM for measuring protein function
  • QF-Pro® signal is generated only when functional events happen
  • QF-Pro® delivers precise and robust quantitative, nanoscopic-resolution data
  • It is a spatial functional platform delivering accurate, accesible, and actionable data

QF-Pro® Benefits

  • Mechanistically quantifies protein functions
  • Spatially maps these functions in tissue and cells
  • Fully compatible with regular laboratory workflows
  • Higher signal-to-noise ratio
  • High specificity and dynamic range (spectroscopic value)
  • Spatial mapping and multiplexing
  • Robust, unbiased and reproducible
  • Proven clinical cut-off values in IO

How does QF-Pro® work?

QF-Pro® Assay Overview

QF-Pro® has modified established technologies (FRET/FLIM) to create a patented assay which is able to spatially quantify protein functions in situ within FPPE tissue samples for the first time at an unprecedented resolution. This is a feat that is unachievable with existing technologies.

Utilisation of Advanced Technologies

QF-Pro® utilises a two-site immunofluorescence-styled assay in conjunction with amplified Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM). Essentially operating as a “biochemical ruler”, QF-Pro® makes 1-10nm measurements within samples to quantify protein functional states. Our patented amplification step has allowed these readouts to perform in patient tissue samples, a feat that has been unachievable with previous FRET-based technologies.

Antibody Labeling Strategy

Two epitopes are labelled simultaneously using species-distinct antibodies. These are then detected with QF-Pro® secondary labelling reagents, one combined with a green chromophore (the donor) and the other to an amplified red chromophore (the acceptor).

Detection

Violet 3.0 and our QF-Pro® software then automatically measures the distance between the donor and acceptor chromophore, based on the amount of energy given from the donor to the acceptor (this is FRET). This allows us to quantify the interaction or post-translational modification of a protein and spatially measure these events across a sample. Violet 3.0 makes FRET imaging easier and more accessible than ever before.

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